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肖君华


肖君华教授



肖君华,1968年10生,生物科学与技术研究所教授,博导,从事遗传学教学科研工作。化学化工与生物工程学院副院长,国际哺乳动物遗传学会成员,中国遗传学会青年委员会委员,上海市遗传学会理事、副秘书长。





研究方向:

1、小鼠性发育相关机制的遗传学研究:利用F2代家系、同类系定位克隆小鼠性发育相关基因,利用转基因小鼠、条件剔除小鼠进行相关基因的功能验证。

2、野生小家鼠遗传资源的挖掘和利用:在全国范围采集了25个地区的野生小家鼠活体,正在建立野生小家鼠来源的1号染色体替换群体。该群体的建立,将大力推动1号染色体已知的400多个QTL的精细定位和功能基因克隆。

3、临床检测方案研究:通过对连接酶检测反应(ligase detective reaction,LDR)的系统研究,建立了LDR-PCR SNP检测系统。上述系统可广泛应用于各类疾病相关基因的检测、个体化用药相关基因的检测、各类病原体基因突变检测和病原体分型鉴别。

近几年承担的科研项目:

1、基因表达、蛋白、组织芯片的研制与开发分项目负责人2002.10—2005.12863基因功能与基因芯片重大专项课题编号2002AA2Z2021

2、中国人女性真性性早熟相关基因的研究2004.1-2006.12国家自然科学基金委员会青年科学基金项目项目批准号30300198

3、小鼠性发育启动调控基因的精细定位和克隆2008,1-2010,12 国家自然科学基金委员会项目,项目批准号:30771191

4、中国野生小家鼠群体遗传结构基本框架的研究国家自然科学基金委员会基金项目 项目批准号:31071090:2011.01-2013.12

近几年论著情况:

*为通讯作者

1、Yuxun Zhou, Yinming Liang, Kai Li, Xiong Bai , Guoqiang Chen, Zhenghong Xing and Junhua Xiao. The phenotypic distribution of quantitative traits in a wild mouse F1 population.Mamm Genome, 23:232–240, 2012

2、Yuxun Zhou, Qinghua Guan, Kai Li, Lingyun Tao, Jianhua Hu, and Junhua Xiao*. Dissection of the maternal effects on puberty onset by embryo transplantation in mouse. J Endocrinolog Invest. 35:676-680, 2012.

3、Jia Li, Lihui Lin, JuanWang, Xia Peng, Yanan Liu, Junhua Xiao, Yuxun Zhou*, Li Li.Quantitative analysis of multiple genes’ expressions based on a novel competitive RT-PCR assay Anal Bioanal Chem. DOI 10.1007/s00216-012-6518-1, 2012

4、Xianhuan Jiang, Jun Gao, Liju Ni, Jianhua Hu, Kai Li, Fengping Sun, Jianyun Xie, Xiong Bo, Chen Gao, Junhua Xiao, Yuxun Zhou*. The complete mitochondrial genome of Microtus fortis calamorum (Arvicolinae, Rodentia) and its phylogenetic analysis. Gene.498: 288-295, 2012

5、Zhourui Tang, Kai Li, Yuxun Zhou*, Zhenxian Xiao, Junhua Xiao, Rui Huang, Guohao Gu. Comparative quantification of human intestinal bacteria based on cPCR and LDR/LCRWorld J Gastroenterology. 18(3): 268-274, 2012

7、陈烨,李凯,周宇荀*,肖君华小鼠基因转录表达分析中内参基因的优选。中国实验动物学报,19(3):197-202, 2011

8、Yuxun Zhou, Qinghua Guan, Kai Li, Lingyun Tao, Jianhua Hu and Junhua Xiao.Dissection of the maternal effects on the puberty onset in mouse by embryo transplantation.J Endocrinol InvestDOI: 10.3275/8125, 2011

9、Ying Zhao, Ting Chen, Yuxun Zhou, Kai Li , Junhua Xiao*, An association study between the genetic polymorphisms within GnRHI, LHβand FSHβgenes and central precocious puberty in Chinese girls.Neuroscience Letters486 : 188–192, 2010

10、Junhua Xiao*, Yinming Liang, Kai Li, Yuxuan Zhou, Wenqian Cai, Yumei Zhou, Ying Zhao, Zhenghong Xing, Guoqiang Chen and Li Jin. A novel strategy for genetic dissection of complex traits: the population of specific chromosome substitution strains from laboratory and wild mice.Mamm GenomeVol 21, 370-376, 2010

11、Liya Zhao, Kai Li, Shimin Bao, Yuxun Zhou, Yinming Liang, Guoji Zhao, Ye Chen and Junhua Xiao.A 1-bp deletion in the γC-crystallin leads to dominant cataracts in mice.Mamm GenomeVol 21, 361-369, 2010

12、Yong-Zhong Wang, Jun-Hua Xiao, Li-Hua Ruan, Hui-Yin Zhang, Min Chen, Xiang-Ke Pu ,Hong-Yu Shen, Guo-Xiang Wu. Detection of the rtA181V/T and rtN236T mutations associated with resistance to adefovir dipivoxil using a ligase detection reaction assay.Clinica Chimica Acta 408 : 70–74,2009

13、王勤熙, 李凯*, 周宇荀, 肖君华. 基于荧光通用引物的多重定量RT-PCR基因表达谱分析平台的构建、优化及其应用. 遗传,31 (5):552―561,2009

14、Jianhui Wang,Minghui Yu,Kai Li, Junhua Xiao,Yuxun Zhou*.Improved PCR-BSP Assay for Multiplex Methylation Pattern Analysis in Minimal Amount of DNA. Mol Biotechnol42(3): 333-340,2009

15、Li Kai, Bei Chen, Yuxun Zhou, Rui Huang, Yinming Liang, Qinxi Wang, Zhenxian Xiao, Junhua Xiao*.Multiplex quantification of 16S rDNA of predominant bacteria group within human fecal samples by polymerase chain reaction-ligase detection reaction (PCR-LDR).Journal of Microbiological Methods, 76: 289-294,2009

16、Wangsheng Zhu, Zhongpeng Fan, Chao Zhang, Zhengxia Guo, Ying Zhao, Yuxun Zhou,Kai Li, Zhenghong Xing, Guoqiang Chen, Yinming Liang, Li Jin, Junhua Xiao*,A Dominant X-Linked QTL Regulating Pubertal Timing in Mice Found by Whole Genome Scanning and Modified Interval-Specific Congenic Strain Analysis PLoS ONE,8(3): e3021,2008

17、MingXing He, Kai Li , JunHua Xiao, YuXun Zhou,Rapid bio-barcode assay for multiplex DNA detection based on capillary DNA AnalyzerJournal of Virological Methods151 :126–131,2008

18、范忠鹏, 朱旺升, 张超, 周宇荀,李凯, 梁银明, 邢正宏, 陈国强, 柏熊, 肖君华*荧光-构象敏感凝胶电泳技术筛查上海市郊区野生小家鼠线粒体编码区SNP位点. 遗传,30(4):475-482,2008

19、X. Luan, H. Yu, X. Wei, Y. Zhou, W. Wang, P. Li, X. Gan, D. Wei and J. Xiao*GPR54 Polymorphisms in Chinese Girls with Central Precocious Puberty Neuroendocrinology86:77–83,2007

20、Xiaohui Luan, Yuxun Zhou,WeiWang, Hong Yu, Pin Li, Xiaohong Gan, DongzhiWei and Junhua Xiao*Association study of the polymorphisms in the KISS1 gene with central precocious puberty in Chinese girlsEuropean Journal of Endocrinology 157 113–118,2007

21、Yuxun Zhou, Wangsheng Zhu, Zhengxia Guo, Ying Zhao, Zijun Song,Junhua Xiao*,Effects of Maternal Nuclear Genome on the Timing of Puberty of offspring in MiceJournal of Endocrinology193:405-412,2007

22、郭正霞,周宇荀,胡亚慧,肖君华*哺乳期营养状况对小鼠性发育启动时间的影响研究华东理工大学学报Vol.33(2):186-189, 2007

23、Zhen-Xian Xiao, Hui-Min Cao, Xiao-Hui Luan, Jian-Long Zhao, Dong-Zhi Wei, Jun-Hua Xiao*Effects of Additives on Efficiency and Specificity of Ligase Detection ReactionMolecular BiotechnologyFebruary Vol 35(2):129-134, 2007

24、Yichuan Xiao, Yuxun Zhou, Jianhui Wang, Gang Wang, Minghui Yu, Jian Jin, Junhua Xiao*, Selection and identification of GnRH promoter binding peptide by phage display-CEMSAJournal of Molecular Recognition.Vol.20(1), Pages 51-57, 2007

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